Product Info

Product Information

Designed for and tested on Roche blocks; a white polypropylene plate with low profile 0.15 ml wells, and a cut corner at H12

This Roche Style plate is designed to achieve optimised assay conditions on the Roche LightCycler® 480.

This particular style of plate is in a low profile 96-well format; perfect for reaction volumes of 10-100 µl. The wells are shorter than "standard" profile wells, which decrease the "dead space" between the heated lid of the thermal cycler and the sample in the well. This eliminates condensation forming on the side wall of the tubes, preventing reduction in PCR volume and increasing the efficiency of the reaction. This is especially recommended for reaction volumes below 20 µl.

Technical Specifications

Plate Width127.70 ± 0.25 mm
Plate Depth85.48 ± 0.25 mm
Plate Height15.60 ± 0.10 mm
Well Depth15.10 ± 0.10 mm
Well Diameter5.50 ± 0.10 mm
Distance to centre of A1 from top edge11.24 ± 0.25 mm
Distance to centre of A1 from left edge14.38 ± 0.25 mm
Pitch (distance between A1 and A2)9.00 mm

Features

Plate Features

  • Optimised for use with the Roche LightCycler® 480
  • Recommended for low volume PCR
  • Ultra-smooth, uniform, thin-walled polypropylene tubes for optimum PCR and real-time (RT, qPCR) results
  • Maximum thermal conductivity for efficient heat transfer and precise thermal cycling
  • Compatible with standard multichannel pipettes
  • 0.15 ml (150 µl) working volume, 0.2 ml (200 µl) max volume
  • Subject to a strict QC procedure which includes a visual check and leak testing of every tube
  • White wells give optimum signal-to-noise ratio when using fluorescent-based assays
  • Raised grid reference to aid well and sample identification
  • Available barcoded

Alternatively, our FrameStar® 2-component PCR plate range offers a plate with the same dimensions, but with the added benefits of a polycarbonate frame that eliminates plate distortion and warping during the PCR process, leading to less evaporation and sample loss. Take a look here.

Barcoding

This plate is available with linear Code 128-format barcodes to aid identification and traceability for your samples. To order your plates with barcodes, simply add /SBC to the end of the ordering code for Standard Single Barcoding, /DBC for Standard Double Barcoding, /TBC for Standard Triple Barcoding, or /QBC for Standard Quadruple Barcoding (see Barcoding for more details).

Compatibility

Compatibility

ROCHE

qPCR CYCLERS

  • LightCycler® 480


For recommended plate options please refer to the Instrument Compatibility Table.


Ordering

Ordering

Code

Description

Plates/Case

4ti-0955 96 Well Semi-Skirted PCR Plate, Roche Style, white polypropylene, designed for use on Roche LightCycler® 480
50 plates
4ti-0955/0560 96 Well Semi-Skirted PCR Plate, Roche Style, white polypropylene, designed for use on Roche LightCycler® 480 + qPCR Adhesive Seal (4ti-0560)
50 plates + 50 seals

Sealing Options

Sealing Options

There are a number of sealing options available to this plate, depending on the application and desired outcome.

Click here to find out more about our Adhesive Seal range >>

Click here to find out more about our Heat Seal range >>

Click here to find out more about our Cap Strip range >>

Click here to find out more about our Lids and Mats >>

See below for a selection:


Related Products