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FrameStar® 384 Well Plates
Features low profile 0.15 ml polypropylene wells, a rigid skirted polycarbonate frame, and a cut corner at H1, now also available as Low Binding plate with ultra-low DNA binding properties
Available in the following colours
"FrameStar® plates led to significantly better results and reduced evaporation compared to standard PCR plates."
The low profile wells of this plate are shorter than "standard" profile wells, which decrease the "dead space" between the heated lid of the thermal cycler and the sample in the well. This eliminates condensation forming on the side wall of the tubes, preventing reduction in PCR volume and increasing the efficiency of the reaction. This is especially recommended for reaction volumes below 20 µl.
The rigid polycarbonate skirt of this design eliminates warping and distortion during PCR, and makes it ideal for use with robotic systems.
Polypropylene (PP) is the best plastic material for PCR tubes as it is chemically inert, resistant to solvents, and well suited for injection moulding - allowing for production of thin-walled tubes for optimum PCR results.
DNA has been shown to bind to PP tubes especially at high ionic strength, despite the very hydrophilic nature of this material. Different PP polymers are used for the production of PCR consumables and as they differ in their characteristics including surface charges, they consequently bind DNA in varying amounts.
DNA binding to PP surfaces has typically only been an issue for reaction tubes and storage vessels but not for PCR/qPCR tubes. DNA sticking to tube walls is either released during denaturation steps and/or remains accessible for amplification.
Nevertheless, due to a progressing volume miniaturisation and with new technologies such as Next Generation Sequencing (NGS), PCR/qPCR tubes are more and more also recommended for other applications which may require ultra-low DNA binding.
Plates of this design with ultra-low DNA binding properties are now available to order under the product code 4ti-LB0960/RIG.Read more about our low binding products here »
Forensic applications demand the highest quality consumables. Avoiding external DNA contamination is of the utmost importance, and as a result high demands are placed upon manufacturers of products for use throughout the workflow.
At 4titude®, we have a stringently controlled cleanroom production facility certified, according to ISO 9001 and ISO 13485, for production of DNA and RNA free products. Following cleanroom production, 4titude®'s consumables for forensic use are packed into ethylene oxide permeable bags and then subjected to ethylene oxide sterilisation. This 2-step processing ensures complete removal of any amplifiable DNA. Read more here »
Plates of this design for forensic use are now available to order under the product code 4ti-OX960C/SBC.
This plate is available with linear Code 128-format barcodes to aid identification and traceability for your samples. To order your plates with barcodes, simply add /SBC to the end of the ordering code for Standard Single Barcoding, /DBC for Standard Double Barcoding, /TBC for Standard Triple Barcoding, or /QBC for Standard Quadruple Barcoding (see Barcoding for more details).
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