"FrameStar® plates led to significantly better results and reduced evaporation compared to standard PCR plates."

Dr. Andreas Dahl, MPI f. Molekulare Genetik, Berlin, Germany

Product Info

Product Information

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Designed for and tested on standard blocks, including ABI® standard blocks; with 0.25 ml polypropylene wells, a rigid semi-skirted polycarbonate frame, and a cut corner at A12

Specifically designed to be directly compatible with all major thermal cyclers, this plate can be used directly in ABI® 96 well instruments without the need for any adaptors.

The rigid FrameStar® 2-component design eliminates warping and distortion during PCR, making it ideal for use with robotic systems. The semi-skirt allows for labelling or barcoding for sample tracking.

For more information on the features and benefits of the FrameStar® range of 2-component PCR plates, please click here »

Technical Specifications

Plate Width124.26 ± 0.25 mm
Plate Depth83.97 ± 0.25 mm
Plate Height20.70 ± 0.25 mm
Well Depth20.20 ± 0.10 mm
Well Diameter5.46 ± 0.10 mm
Distance to centre of A1 from top edge10.495 ± 0.25 mm
Distance to centre of A1 from left edge12.63 ± 0.25 mm
Pitch (distance between A1 and A2)9.00 mm

Features

Plate Features

  • Cut Corner at A12 - for a variety of plate with a cut corner at H1, click here »
  • Perfect for ABI® thermal cyclers & sequencers
  • Recommended for low volume PCR
  • Rigid polycarbonate frame adds to the mechanical stability of the plate - as a result thermal expansion and sample evaporation is reduced significantly, leading to an improved consistency in PCR results
  • Ultra-smooth, uniform, thin-walled polypropylene tubes for optimum PCR and real-time (RT, qPCR) results
  • Improved seal integrity due to thermal stability of frame
  • Compatible with standard multichannel pipettes
  • Ideal for use with robotic systems
  • 0.25 ml (250 µl) working volume, 0.3 ml (300 µl) max volume
  • 6 frame colour options for the clear well variety - purple, blue, clear, green, red, and black
  • Also available as a black frame with white wells for optimum signal-to-noise ratio when using fluorescent based assays
  • Raised and colour differentiated grid reference to aid well and sample identification
  • Available barcoded


Now Available - Low Binding Plates with Ultra-Low DNA Binding Properties

Sensitive applications such as Next Generation Sequencing sample preparation applications demand the highest quality consumables. Low binding polypropylene tubes allow for multiple incubation and transfer steps without sample loss.

Polypropylene (PP) is the best plastic material for PCR tubes as it is chemically inert, resistant to solvents, and well suited for injection moulding - allowing for production of thin-walled tubes for optimum PCR results.

DNA has been shown to bind to PP tubes especially at high ionic strength, despite the very hydrophobic nature of this material. Different PP polymers are used for the production of PCR consumables and as they differ in their characteristics including surface charges, they consequently bind DNA in varying amounts.

DNA binding to PP surfaces has typically only been an issue for reaction tubes and storage vessels but not for PCR/qPCR tubes. DNA sticking to tube walls is either released during denaturation steps and/or remains accessible for amplification.

Nevertheless, due to a progressing volume miniaturisation and with new technologies such as Next Generation Sequencing (NGS), PCR/qPCR tubes are more and more also recommended for other applications which may require ultra-low DNA binding.

Plates of this design with ultra-low DNA binding properties are now available to order under the product code 4ti-LB0770/C.

Read more about our low binding products here »


  • Low-binding feature results from selected low-bind polymers, no coating is used to achieve the binding characteristics - No contamination or modification of the samples
  • Tested under a broad temperature range - Maximum DNA recovery after low temperature storage and high temperature incubation
  • No DNA loss during incubation steps and sample transfer in NGS sample prep and library construction - Ideally suited for sensitive applications with ultra-low DNA input

Now Available - Frosted Wells for use with ABI®/LifeTechnologies® qPCR Instruments

While clear wells are the best for sample visibility and optical appeal, white wells increase the signal-to-noise ratio by maximising reflect of light in fluorescent based assays used in real-time PCR and qPCR. This leads to a better sensitivity and reproducibility from a wide range of qPCR instruments from most manufacturers.

However, white well PCR plates may lead to software related problems with ABI®/LifeTechnologies® cyclers. For this reason the manufacturer recommends the use of plates with frosted wells as an alternative to white wells. Although the frosting does not provide the same sensitivity as white wells, readings are still considerably higher than those achieved with standard clear wells.

In response to this, we at 4titude® have developed a frosted well variety of the FrameStar® 96 Well Semi-Skirted Plate to offer ABI®/LifeTechnologies® instrument users the time and money-saving advantages of FrameStar®. And as the signal levels are directly comparable with ABI®/LifeTechnologies® plates, there's no need for users to recalibrate their instruments.

Find these plates in the Order tab under product code 4ti-0772.

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Frosted wells compared to clear wells

Now Available - Ethylene Oxide Treated Plates

Forensic applications demand the highest quality consumables. Avoiding external DNA contamination is of the utmost importance, and as a result high demands are placed upon manufacturers of products for use throughout the workflow.

At 4titude®, we have a stringently controlled cleanroom production facility certified, according to ISO 9001 and ISO 13485, for production of DNA and RNA free products. Following cleanroom production, 4titude®'s consumables for forensic use are packed into ethylene oxide permeable bags and then subjected to ethylene oxide sterilisation. This 2-step processing ensures complete removal of any amplifiable DNA. Read more here »

Plates of this design for forensic use are now available to order under the product code 4ti-OX770C/SBC.

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Barcoding

This plate is available with linear Code 128-format barcodes to aid identification and traceability for your samples. To order your plates with barcodes, simply add /SBC to the end of the ordering code for Standard Single Barcoding, /DBC for Standard Double Barcoding, /TBC for Standard Triple Barcoding, or /QBC for Standard Quadruple Barcoding (see Barcoding for more details).

Compatibility

Compatibility

STANDARD THERMAL CYCLERS

ABI/LIFE TECHNOLOGIES

  • GeneAmp® 2700, 2720, 9600
  • GeneAmp® 9700
  • Veriti 0.2ml (96 well block)

BIOMETRA

  • T1 Thermocycler
  • TGradient

BIO-RAD

  • C1000
  • iCycler™
  • MyCycler™
  • S1000
  • T100

BIO-RAD MJ RESEARCH

  • PTC100™ (96 well block only)
  • DNA Engine™, DNA Dyad™, DNA Tetrad™

EPPENDORF

  • MasterCycler®
  • MasterCycler® EP Gradient

MWG

  • Primus 96
  • The Q Lifecycler

PEQLAB

  • peqSTAR 96

SENSOQUEST

  • labcycler

DNA SEQUENCERS

ABI/LIFE TECHNOLOGIES

  • 310, 3100 Genetic Analyser
  • 3130 Genetic Analyser
  • 3500 Genetic Analyser
  • 3500XL
  • 3700, 3730, 3730XL DNA Analyser


QPCR CYCLERS

ABI/LIFE TECHNOLOGIES

  • 7000, 7300, 7500, 7700, 7900
  • QuantStudio™
  • ViiA7™

BIO-RAD

  • iCycler™ IQ, IQ™ 4, IQ™ 5
  • MyiQ

EPPENDORF

  • Mastercycler™ ep realplex

STRATAGENE/AGILENT

  • Mx3000P™
  • Mx3005P™
  • Mx4000™

TECHNE

  • Quantica

Ordering

Ordering

Code

Description

Plates/Case

4ti-0770 FrameStar® 96 Well Semi-Skirted PCR Plate, clear wells, purple frame
50 plates
4ti-0770/B FrameStar® 96 Well Semi-Skirted PCR Plate, clear wells, blue frame 50 plates
4ti-0770/C FrameStar® 96 Well Semi-Skirted PCR Plate, clear wells, clear frame 50 plates
4ti-0770/C/10P FrameStar® 96 Well Semi-Skirted PCR Plate, clear wells, clear frame 10 plates
4ti-LB0770/C FrameStar® 96 Well Semi-Skirted PCR Plate, clear wells, clear frame, Low Binding 50 plates
4ti-0770/G FrameStar® 96 Well Semi-Skirted PCR Plate, clear wells, green frame 50 plates
4ti-0770/R
FrameStar® 96 Well Semi-Skirted PCR Plate, clear wells, red frame 50 plates
4ti-0770/X FrameStar® 96 Well Semi-Skirted PCR Plate, clear wells, black frame 50 plates
4ti-OX770C/SBC FrameStar® 96 Well Semi-Skirted PCR Plate for Forensic Use, clear wells, clear frame,
Ethylene Oxide treated, single barcode
20 plates
4ti-0771 FrameStar® 96 Well Semi-Skirted qPCR Plate, white wells, black frame 50 plates
4ti-0772 FrameStar® 96 Well ABI® Optical Style plate, frosted wells, clear frame 50 plates
4ti-0772/10P FrameStar® 96 Well ABI® Optical Style plate, frosted wells, clear frame 10 plates
4ti-0289 FrameStar® 96 Lid, non-sterile, cut corner at A12, without condensation rings, for use with the 4ti-0770 range 50 lids

Sealing Options

Sealing Options

There are a number of sealing options available to this plate, depending on the application and desired outcome.

Click here to find out more about our Adhesive Seal range >>

Click here to find out more about our Heat Seal range >>

Click here to find out more about our Cap Strip range >>

Click here to find out more about our Lids and Mats >>

See below for a selection:


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