"FrameStar® plates led to significantly better results and reduced evaporation compared to standard PCR plates."

Dr. Andreas Dahl, MPI f. Molekulare Genetik, Berlin, Germany

Product Info

Product Information

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Designed for and tested on Roche blocks; with low profile 0.15 ml polypropylene wells, a rigid semi-skirted polycarbonate frame, and a cut corner at H12


Our FrameStar® Roche Style plates are designed to achieve optimised assay conditions on the Roche LightCycler® 480.

This particular style of plate is in a low profile 96-well format; perfect for reaction volumes of 10-100 µl. The wells are shorter than "standard" profile wells, which decrease the "dead space" between the heated lid of the thermal cycler and the sample in the well. This eliminates condensation forming on the side wall of the tubes, preventing reduction in PCR volume and increasing the efficiency of the reaction. This is especially recommended for reaction volumes below 20 µl.

The semi-skirted frame allows for labelling or barcoding.

Technical Specifications

Plate Width127.70 ± 0.25 mm
Plate Depth85.48 ± 0.25 mm
Plate Height15.60 ± 0.25 mm
Well Depth15.10 ± 0.10 mm
Well Diameter5.50 ± 0.10 mm
Distance to centre of A1 from top edge11.24 ± 0.25 mm
Distance to centre of A1 from left edge14.38 ± 0.25 mm
Pitch (distance between A1 and A2)9.00 mm

Features

Plate Features

  • Compatible with the Roche LightCycler® 480
  • Recommended for low volume PCR
  • Rigid polycarbonate frame adds to the mechanical stability of the plate - as a result thermal expansion and sample evaporation is reduced significantly, leading to an improved consistency in PCR results
  • Ultra-smooth, uniform, thin-walled polypropylene tubes for optimum PCR and real-time (RT, qPCR) results
  • Improved seal integrity due to thermal stability of frame
  • Compatible with standard multichannel pipettes
  • Ideal for use with robotic systems
  • 0.15 ml (150 µl) working volume, 0.2 ml (200 µl) max volume
  • Standard plate comes with clear polypropylene wells and a clear polycarbonate frame
  • Also available with white wells for optimum signal-to-noise ratio when using fluorescent based assays
  • Raised and colour differentiated grid reference to aid well and sample identification
  • Available barcoded

Barcoding

This plate is available with linear Code 128-format barcodes to aid identification and traceability for your samples. To order your plates with barcodes, simply add /SBC to the end of the ordering code for Standard Single Barcoding, /DBC for Standard Double Barcoding, /TBC for Standard Triple Barcoding, or /QBC for Standard Quadruple Barcoding (see Barcoding for more details).

Compatibility

Compatibility

qPCR CYCLERS

ROCHE

  • LightCycler® 480
  • LightCycler® 96

Ordering

Ordering

Code

Description

Plates/Case

4ti-0950/C FrameStar® 96 Well Roche Style PCR Plate, clear wells, clear frame
50 plates
4ti-0951 FrameStar® 96 Well Roche Style qPCR Plate, white wells, clear frame50 plates
4ti-0951/0565 FrameStar® 96 Well Roche Style qPCR Plate, white wells, clear frame + Q-Stick™ Adhesive Seals (4ti-0565)50 plates + 50 seals
4ti-0952 FrameStar® 96 Well Roche Style qPCR Plate, white wells, clear frame + qPCR Adhesive Seal (4ti-0560)50 plates + 50 seals
4ti-0953
FrameStar® 96 Well Roche Style PCR Plate, clear wells, clear frame + qPCR Adhesive Seal (4ti-0560) 50 plates + 50 seals

Sealing Options

Improved Sealing

There are a number of sealing options available to this plate, depending on the application and desired outcome.
    See below for a selection:


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