"FrameStar® plates led to significantly better results and reduced evaporation compared to standard PCR plates."

Dr. Andreas Dahl, MPI f. Molekulare Genetik, Berlin, Germany

Product Info

Product Information

#

Low profile, 0.1 ml polypropylene wells, semi-skirted polycarbonate frame, cut corner H12; designed for use on Roche LightCycler® 480 with 96 well block

Our FrameStar® Roche Style plates are designed to achieve optimised assay conditions on the Roche LightCycler® 480.

This particular style of plate is in a low profile 96-well format; perfect for reaction volumes of 10-100 µl. The wells are shorter than "standard" profile wells, which decrease the "dead space" between the heated lid of the thermal cycler and the sample in the well. This eliminates condensation forming on the side wall of the tubes, preventing reduction in PCR volume and increasing the efficiency of the reaction. This is especially recommended for reaction volumes below 20 µl.

The semi-skirted frame allows for labelling or barcoding.

Technical Specifications

Plate Width127.70 ± 0.25 mm
Plate Depth85.48 ± 0.25 mm
Plate Height15.60 ± 0.25 mm
Well Depth15.10 ± 0.10 mm
Well Diameter5.50 ± 0.10 mm
Distance to centre of A1 from top edge11.24 ± 0.25 mm
Distance to centre of A1 from left edge14.38 ± 0.25 mm
Pitch (distance between A1 and A2)9.00 mm

Features

Plate Features

  • Compatible with the Roche LightCycler® 480
  • Recommended for low volume PCR
  • Rigid polycarbonate frame adds to the mechanical stability of the plate - as a result thermal expansion and sample evaporation is reduced significantly, leading to an improved consistency in PCR results
  • Ultra-smooth, uniform, thin-walled polypropylene tubes for optimum PCR and real-time (RT, qPCR) results
  • Improved seal integrity due to thermal stability of frame
  • Compatible with standard multichannel pipettes
  • Ideal for use with robotic systems
  • <0.1 ml (100 µl) working volume, 0.2 ml (200 µl) total well capacity
  • Standard plate comes with clear polypropylene wells and a clear polycarbonate frame
  • Also available with white wells for optimum signal-to-noise ratio when using fluorescent based assays
  • Raised and colour differentiated grid reference to aid well and sample identification
  • Available barcoded

Barcoding

This plate is available with linear Code 128-format barcodes to aid identification and traceability for your samples. To order your plates with barcodes, simply add /SBC to the end of the ordering code for Standard Single Barcoding, /DBC for Standard Double Barcoding, /TBC for Standard Triple Barcoding, or /QBC for Standard Quadruple Barcoding (see Barcoding for more details).

Compatibility

Compatibility

MANUFACTURER

BLOCK TYPE

BLOCK NAME

ROCHE qPCR Cyclers (96 well block) LC96 | LC480


For recommended plate options please refer to the Instrument Compatibility Table.


Ordering

Ordering

FrameStar® 96 Well Semi-Skirted PCR Plate, Roche Style

Low profile, 0.1 ml PP wells, PC frame, cut corner H12, working volume: <100 µl, total well capacity: 200 µl

Code

Description

Plates/Case

4ti-0950/C clear wells, clear frame
50 plates
4ti-0951 white wells, clear frame 50 plates

Code Combi Pack

Plates and Seals/Case

4ti-0952 4ti-0951 + qPCR Seal (4ti-0560) 50 plates + 50 seals
4ti-0953
4ti-0950/C + qPCR Seal (4ti-0560) 50 plates + 50 seals
4ti-0951/0565 4ti-0951 + Q-Stick™ qPCR Seal (4ti-0565) 50 plates + 50 seals

Sealing Options

Improved Sealing

There are a number of sealing options available to this plate, depending on the application and desired outcome.

Click here to find out more about our Adhesive Seal range >>

Click here to find out more about our Heat Seal range >>

Click here to find out more about our Cap Strip range >>

Click here to find out more about our Lids and Mats >>

See below for a selection:


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