"FrameStar® plates led to significantly better results and reduced evaporation compared to standard PCR plates."

Dr. Andreas Dahl, MPI f. Molekulare Genetik, Berlin, Germany

Product Info

Product Information

Technical Drawing of FrameStar® 96 Well Semi-Skirted PCR Plate With Upstand, ABI® Style

Standard profile, 0.2 ml polypropylene wells, semi-skirted polycarbonate frame with upstand, cut corner A12; designed for use on ABI® instruments

This ABI® Style PCR plate offers the benefits of our 2-component design to ABI® standard block users. This design combines the advantages of ultra-thin wall polypropylene tubes for optimum PCR results with a rigid polycarbonate skirt and deck for the highest thermal stability.

For more information on the features and benefits of the FrameStar® range, please click here »

We recommend this semi-skirted plate for use with ABI® thermal cyclers and sequencers; it can be used directly with ABI® instruments with no adaptors necessary. The only case where this is not true is with the ABI Fast Block thermal cyclers; in which case using our FrameStar® FastPlate is recommended instead - found here.

Technical Specifications

Plate Width124.26 ± 0.25 mm
Plate Depth83.97 ± 0.25 mm
Plate Height23.20 ± 0.25 mm
Well Depth20.20 ± 0.10 mm
Well Diameter5.46 ± 0.10 mm
Distance to centre of A1 from top edge10.495 ± 0.25 mm
Distance to centre of A1 from left edge12.63 ± 0.25 mm
Pitch (distance between A1 and A2)9.00 mm

Features

Plate Features

  • Ideal for use with ABI® thermal cyclers & sequencers
  • Recommended for low volume PCR
  • Rigid polycarbonate frame adds to the mechanical stability of the plate - as a result thermal expansion and sample evaporation is reduced significantly, leading to an improved consistency in PCR results
  • Ultra-smooth, uniform, thin-walled polypropylene tubes for optimum PCR and real-time quantitative PCR (RT-qPCR) results
  • Improved seal integrity due to thermal stability of frame
  • Compatible with standard multichannel pipettes
  • Ideal for use with robotic systems
  • <0.2 ml (200 µl) working volume, 0.3 ml (300 µl) total well capacity
  • 6 frame colour options for the clear well variety - purple, blue, clear, green, red, and black
  • Also available as a black frame with white wells for optimum signal-to-noise ratio when using fluorescent based assays
  • Raised and colour differentiated grid reference to aid well and sample identification
  • Available barcoded

Now Available - Ethylene Oxide Treated Plates

Forensic applications demand the highest quality consumables. Avoiding external DNA contamination is of the utmost importance, and as a result high demands are placed upon manufacturers of products for use throughout the workflow.

At 4titude®, we have a stringently controlled cleanroom production facility certified, according to ISO 9001 and ISO 13485, for production of DNA and RNA free products. Following cleanroom production, 4titude®'s consumables for forensic use are packed into ethylene oxide permeable bags and then subjected to ethylene oxide sterilisation. This 2-step processing ensures degradation of amplifiable DNA. Read more here »

Plates of this design for forensic use are now available to order under the product code 4ti-OX730C/SBC.

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Barcoding

This plate is available with linear Code 128-format barcodes to aid identification and traceability for your samples. To order your plates with barcodes, simply add /SBC to the end of the ordering code for Standard Single Barcoding, /DBC for Standard Double Barcoding, /TBC for Standard Triple Barcoding, or /QBC for Standard Quadruple Barcoding (see Barcoding for more details).

Compatibility

Compatibility

MANUFACTURER

BLOCK TYPE

INSTRUMENT

ABI® / LIFE TECHNOLOGIES / THERMO FISHER SCIENTIFIC Thermal Cyclers (96 well standard block) Verity | Proflex | Simpliamp | GeneAmp® 2700 / 2720 / 9600 / 9700
qPCR Cyclers (96 well standard block) 7000 | 7300 | 7500 | 7700 | 7900 HT | QuantStudio™ 3 / 5 / 6 / 7 / 12K | ViiA7™
Sequencers (96 well block) 3100 | 3130XL | 3500 | 3500XL | 3730 | 3730XL
AGILENT / STRATAGENE qPCR Cyclers (96 well block) Mx4000™
SENSOQUEST Thermal Cyclers (96 well block) Labcycler


For recommended plate options please refer to the Instrument Compatibility Table.


Ordering

Ordering

FrameStar® 96 Well Semi-Skirted PCR Plate With Upstand, ABI® Style

Standard profile, 0.2 ml PP wells, PC frame with upstand, cut corner A12, working volume: <200 µl, total well capacity: 300 µl

Code

Description

Plates/Case

4ti-0730 clear wells, purple frame
50 plates
4ti-0730/B clear wells, blue frame 50 plates
4ti-0730/C clear wells, clear frame 50 plates
4ti-0730/C/10P clear wells, clear frame 10 plates
4ti-0730/G clear wells, green frame 50 plates
4ti-0730/R
clear wells, red frame 50 plates
4ti-0730/X clear wells, black frame 50 plates
4ti-0731 white wells, black frame 50 plates
4ti-OX730C/SBC 4ti-0730/C for forensic use, ethylene oxide treated, single barcoded 20 plates

Combi Pack Code

Description

Plates & Seals/Case

4ti-0730/C/0565 4ti-0730/C + Q-Stick™ qPCR Seal (4ti-0565) 50 plates + 50 seals

Sealing Options

Sealing Options

There are a number of sealing options available to this plate, depending on the application and desired outcome.

Click here to find out more about our Adhesive Seal range >>

Click here to find out more about our Heat Seal range >>

Click here to find out more about our Cap Strip range >>

Click here to find out more about our Lids and Mats >>

See below for a selection:


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