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FrameStar® 384 Well Plates
Designed for and tested on ABI® Fast blocks; with low profile 0.15 ml polypropylene wells, a rigid semi-skirted polycarbonate frame, and a cut corner at A1
Available in the following colours
"FrameStar® plates led to significantly better results and reduced evaporation compared to standard PCR plates."
This semi-skirted low profile plate is recommended for use with ABI® Fast block thermal cyclers.
The low profile wells of this plate are shorter than "standard" profile wells, which decrease the "dead space" between the heated lid of the thermal cycler and the sample in the well. This eliminates condensation forming on the side wall of the tubes, preventing reduction in PCR volume and increasing the efficiency of the reaction. This is especially recommended for reaction volumes below 20 µl.
The rigid polycarbonate skirt of this design eliminates warping and distortion during PCR, and makes it ideal for use with robotic systems. Its skirt also allows for labelling or barcoding.
While clear wells are the best for sample visibility and optical appeal, white wells increase the signal-to-noise ratio by maximising reflect of light in fluorescent based assays used in real-time PCR and qPCR. This leads to a better sensitivity and reproducibility from a wide range of qPCR instruments from most manufacturers.
However, white well PCR plates may lead to software related problems with ABI®/Life Technologies® cyclers. For this reason the manufacturer recommends the use of plates with frosted wells as an alternative to white wells. Although the frosting does not provide the same sensitivity as white wells, readings are still considerably higher than those achieved with standard clear wells.
In response to this, we at 4titude® have developed a frosted well variety of the FrameStar® FastPlate 96 well to offer ABI®/Life Technologies® instrument users the time and money-saving advantages of FrameStar®. And as the signal levels are directly comparable with ABI®/Life Technologies® plates, there's no need for users to recalibrate their instruments.
Find these plates in the Order tab under product code 4ti-0912.
Frosted wells compared to clear wells
This plate is available with linear Code 128-format barcodes to aid identification and traceability for your samples. To order your plates with barcodes, simply add /SBC to the end of the ordering code for Standard Single Barcoding, /DBC for Standard Double Barcoding, /TBC for Standard Triple Barcoding, or /QBC for Standard Quadruple Barcoding (see Barcoding for more details).
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