"FrameStar® plates led to significantly better results and reduced evaporation compared to standard PCR plates."

Dr. Andreas Dahl, MPI f. Molekulare Genetik, Berlin, Germany

Product Info

Product Information

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Designed to fit standard blocks; with 30 µl polypropylene wells, a skirted polycarbonate frame, and a cut corner at A24


Our FrameStar® 384 Well Plates are designed for high-throughput PCR. They are compatible with the majority of 384 well block PCR, qPCR and sequencing instruments, and the rigid 2-component design eliminates warping and distortion during PCR making it ideal for use with robotic systems.

The skirt allows for labelling or barcoding.

Technical Specifications

Plate Width127.76 ± 0.25 mm
Plate Depth85.48 ± 0.25 mm
Plate Height10.60 ± 0.25 mm
Well Depth9.60 ± 0.10 mm
Well Diameter3.10 ± 0.10 mm
Distance to centre of A1 from top edge8.99 ± 0.25 mm
Distance to centre of A1 from left edge12.13 ± 0.25 mm
Pitch (distance between A1 and A2)4.50 mm

Features

Plate Features

  • Recommended for low volume PCR
  • Rigid polycarbonate frame adds to the mechanical stability of the plate - as a result thermal expansion and sample evaporation is reduced significantly, leading to an improved consistency in PCR results
  • Ultra-smooth, uniform, thin-walled polypropylene tubes for optimum PCR and real-time (RT, qPCR) results
  • Improved seal integrity due to thermal stability of the frame
  • Compatible with standard multichannel pipettes
  • Ideal for use with robotic systems
  • 30 µl working volume, 55 µl max volume
  • Compatible with majority of 384 well block PCR, qPCR and sequencing instruments
  • 6 frame colour options for the clear well variety - purple, blue, clear, green, red, and black
  • Also available as a black frame with white wells for optimum signal-to-noise ratio when using fluorescent based assays
  • Raised and colour differentiated grid reference to aid well and sample identification
  • Stackable
  • Available barcoded

Now Available - Frosted Wells for use with ABI®/Life Technologies® qPCR Instruments

While clear wells are the best for sample visibility and optical appeal, white wells increase the signal-to-noise ratio by maximising reflect of light in fluorescent based assays used in real-time PCR and qPCR. This leads to a better sensitivity and reproducibility from a wide range of qPCR instruments from most manufacturers.

However, white well PCR plates may lead to software related problems with ABI®/Life Technologies® cyclers. For this reason the manufacturer recommends the use of plates with frosted wells as an alternative to white wells. Although the frosting does not provide the same sensitivity as white wells, readings are still considerably higher than those achieved with standard clear wells.

In response to this, we at 4titude® have developed a frosted well variety of the FrameStar® 384 well plate to offer ABI®/Life Technologies® instrument users the time and money-saving advantages of FrameStar®. And as the signal levels are directly comparable with ABI®/Life Technologies® plates, there's no need for users to recalibrate their instruments.

Find these plates in the Order tab under product code 4ti-0387.

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Frosted wells compared to clear wells

Barcoding

This plate is available with linear Code 128-format barcodes to aid identification and traceability for your samples. To order your plates with barcodes, simply add /SBC to the end of the ordering code for Standard Single Barcoding, /DBC for Standard Double Barcoding, /TBC for Standard Triple Barcoding, or /QBC for Standard Quadruple Barcoding (see Barcoding for more details).

Compatibility

Compatibility

STANDARD THERMAL CYCLERS

ABI / LIFE TECHNOLOGIES

  • GeneAmp® 9700
  • Veriti (384-well block)

BIOMETRA

  • T1 Thermocycler
  • Tprofessional (Standard) 384

BIO-RAD

  • C1000
  • S1000

BIO-RAD MJ RESEARCH

  • DNA Engine™, DNA Dyad™, DNA Tetrad™

CORBETT RESEARCH

  • (Qiagen) Palm Cycler 384

EPPENDORF

  • M384
  • MWG

  • Primus 384

PEQLAB

  • peqSTAR 384

SENSOQUEST

  • labcycler

TECHNE

  • Flexigene
  • TC412/512
  • Touchgene Gradient

THERMO FISHER SCIENTIFIC

  • MultiBlock System, MBS


DNA SEQUENCERS

ABI / LIFE TECHNOLOGIES

  • 3130 Genetic Analyser
  • 3500XL
  • 3700, 3730, 3730XL DNA Analyser

AMERSHAM

  • MegaBACE™ 4000


qPCR CYCLERS

ABI / LIFE TECHNOLOGIES

  • 7900 HT
  • QuantStudio™
  • ViiA7™

BIO-RAD

  • CFX 384

BIOMETRA

  • Toptical Thermalcycler

Ordering

Ordering

Code

Description

Plates/Case

4ti-0384 FrameStar® 384 Well PCR Plate, clear wells, purple frame 50 plates
4ti-0384/B FrameStar® 384 Well PCR Plate, clear wells, blue frame
50 plates
4ti-0384/C FrameStar® 384 Well PCR Plate, clear wells, clear frame
50 plates
4ti-0384/G FrameStar® 384 Well PCR Plate, clear wells, green frame
50 plates
4ti-0384/R FrameStar® 384 Well PCR Plate, clear wells, red frame
50 plates
4ti-0384/X FrameStar® 384 Well PCR Plate, clear wells, black frame
50 plates
4ti-0385 FrameStar® 384 Well qPCR Plate, white wells, black frame
50 plates
4ti-0386 FrameStar® 384 Well PCR Plate, black wells, black frame
50 plates
4ti-0387 FrameStar® 384 Well ABI®/Life Technologies® Style Optical qPCR Plate, frosted wells, clear frame 50 plates

Sealing Options

Sealing Options

There are a number of sealing options available to this plate, depending on the application and desired outcome.

    See below for a selection:


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