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4ti-0910/C

FrameStar® 96 Well ABI FastPlate Style PCR and qPCR Plates

Designed for and tested on ABi fast blocks. Semi-skirted, low profile 0.1 ml polypropylene wells, a clear polycarbonate frame, and a cut corner at A1

 

This semi-skirted low profile plate is recommended for ABi™ Fast Block thermal cyclers.  Low profile plates have shorter wells than standard profile plates, decreasing the "dead space" between the heated lid of the thermal cycler and the sample.  This eliminates any chance of condensation forming on the side walls of the tubes, both preventing a reduction in PCR volume, and increasing the efficiency of the reaction. Low profile products are especially recommended for use with reaction volumes below 20 µl.  The rigid 2-component design eliminates warping and distortion during PCR making it ideal for use with robotic systems.  The semi-skirt allows for labelling or barcoding.

 

 

Plate Features

 

  • Compatible with the ABi Fast BLock thermal cyclers

 

  • Recommended for low volume PCR

 

  • Rigid Polycarbonate frame adds to the mechanical stability of the plate - as a result thermal expansion and sample evaporation is reduced significantly, leading to an improved consistency in PCR results

 

  • Ultra-smooth, uniform, thin-walled polypropylene tubes for optimum PCR and real-time (RT, qPCR) results

 

  • Improved seal integrity due to thermal stability of frame

 

  • Compatible with standard multichannel pipettes

 

  • Ideal for use with robotic systems

 

  • 0.1 ml (100 µl) working volume, 0.2 ml (200µl) total tube volume

 

  • Available with white wells for optimum signal-to-noise ratio when using fluorescent-based assays

 

  • Raised and colour differentiated grid reference to aid well and sample identification

 

  • Available barcoded

 

To read more about the features and benefits of our 2-component FrameStar® plate range, click here»

 

 

Now with frosted wells for ABi/LifeTech qPCR instruments

 

While clear wells are the best for sample visibility and optical appeal, white wells increase the signal-to-noise ratio by maximising reflection of light in fluorescent based assays used in real-time PCR (qPCR). This leads to a better sensitivity and reproducibility from a wide range of qPCR instruments from most manufacturers.

 

However, white well PCR plates may lead to software related problems with ABi/LifeTech cyclers.  For this reason the manufacturer recommends the use of plates with frosted wells as an alternative to white wells.  Although the frosting does not provide the same sensitivity as white wells, readings are still considerably higher than those achieved with standard clear wells.

 

In response to this, we at 4titude® have developed a frosted well variant of the FrameStar® FastPlate 96 well to offer ABi/LifeTech instrument users the time and money saving advantages of FrameStar®.  And as the signal levels are directly comparable with ABi/LifeTech plates, there's no need for users to recalibrate their instruments. Find it below with the product code 4ti-0912.

 

 

frosted wells compared with clear wells

 

 

Barcoding

 

To order your plates with barcodes, simply add /SBC to the end of the ordering code for Standard Single Barcoding, or /DBC for Standard Double Barcoding (please see Barcoding for more details).

Code

Description

Plates/Case

4ti-0910/C

4ti-0910/C/10P

4ti-0910/C/0565

 

4ti-0911

4ti-0912

4ti-0912/10P

FrameStar® 96 Well ABI FastPlate PCR Plate, semi-skirted, clear wells, clear frame

FrameStar® 96 Well ABI FastPlate PCR Plate, semi-skirted, clear wells, clear frame

FrameStar® 96 Well ABI FastPlate PCR Plate, semi-skirted, clear wells, clear frame

+ Q-Stick Optically Clear Adhesive Seals (4ti-0565)

FrameStar® 96 Well ABI FastPlate qPCR Plate, semi-skirted, white wells, clear frame

FrameStar® 96 Well ABI FastPlate qPCR Plate, semi-skirted, frosted wells, clear frame

FrameStar®96 Well ABI FastPlate qPCR Plate, semi-skirted, frosted wells, clear frame

50

10

50

+ 50 Seals

50

50

10

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"Reducing the SyBr Green consumption by 40% means considerable cost savings to us."

Dr Carolin Deiner, Institut für Veterinär-Physiologie, Berlin, Germany