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4LAB™:
Automated Low Volume Liquid Handling

 

4titude's® 4LAB™ is an automated, high-precision pipetting system specifically designed for low-volume liquid handling.  For acceptable PCR results, small volumes of reagents need to be dispensed accurately and consistently, which is tedious, time consuming and prone to human error.

It is available as a 4-position and a 6-position workstation.

4LAB™ is designed to replace manual PCR/qPCR sample preparation. Accuracy, precision and consistency are guaranteed, and reagent wastage is reduced. Unlike complex, multi-purpose robotic systems, our instrument was designed for the researcher without prior robotic experience.

Intuitive set up and programming will save your laboratory time and money straight away.

 

 

Easy and Convenient to Use

 

  • PC software can be mastered in an hour. 
  • No trained technician required.
  • Built-in set-up protocols can be quickly modified and transferred via USB memory stick.
  • Single and 8-channel, 50µl or 200µl automated pipetting modules can be changed without tools. 
  • 4 interchangeable standard microplate / tip rack adapters and 2 interchangeable reagent vessel adapters available.
  • Other applications include sequencing set-up, HLA typing and SNP detection, etc.

 

 

Affordable Solution

 

  • The most affordable automated pipetting system available.
  • Uses tips that are compatible with Beckman® Biomek® NX, FX & 3000.
  • Cooling block adapters keep sensitive reagents / samples at 7°C for over an hour.
  • Save reagent costs by human error reduction and using low volume PCR plates.

 

 

Easy to Service

 

  • The automated pipetting modules are simple to remove and can be returned for service and calibration.
  • PC software is upgraded via the internet.
  • The entire system is compact and light-weight.

 

 

Accurate and Precise

 

  • Each automated pipetting module is calibrated to ISO-8655 standards.
  • Excellent results for qPCR standard curve and replicates (Figure 1).
  • Better Precision of pipetting results than manual pipetting (Figure 2).

Figure 1

Figure 1: Excellent Serial Dilution Results

7µl NIH3T3 Cell cDNA sample is diluted in 21µl water at 1:4 ratio for 4 times. Roche® LightCycler® 480 real-time thermal cycler and Finnzyme DyNAmo® Flash SYBR® Green qPCR kit (F-415L) were used.

Figure 2: Better Precision Results than Manual Pipetting

Target: Human GAPDH (top curve) was amplified in replicates of 4. 18µl (master-mix) and 2µl (cDNA) were pipetted into 20µl reaction volume. Roche  LightCycler 480 real-time thermal cycler and Invitrogen   Platinum Taq DNA Ploymerase were used.

 

Performance

50µl Tip on single or 8-channel pipetting module

 

1µl

50µl

Accuracy (Rel.)

±7%

±1%

Precision (Rel. CV)

≤ 7.5%

≤ 0.4%

 

200µl Tip on single or 8-channel pipetting module

 

10µl

200µl

Accuracy (Rel.)

±3%

±0.8%

Precision (Rel. CV)

≤ 1%

≤ 0.15%

 

Note: According to ISO-8655 standards (Gravimetric method), APM is calibrated in temperature (21-25°C, ±0.5°C) and humidity (60-90%) controlled environment. Twice-distilled water, robotic tips and microbalance were used.


 

UV light to eliminate DNA contamination

Note: picture shows optional modifications

 


 

 

HEPA filter removes 99.97% of particles 0.3 micrometres or larger

Note: picture shows optional modifications

specifications

Worktable Capacity:
2-3 x 96/384 PCR plates
1-2 x 96 tip racks (50/200µl)

Reagent area 1:
8 x 1.5/2ml microcentrifuge tubes

Reagent area 2:
6 x 2ml storage tubes (free standing) and 1 x 5ml bottle


Function:
Liquid transfer (LT)
Multiple Dispense (MD)
Serial Dilution (SD)
Hold (Pause)
Mixing (MIX)
Loop

 

Automated Pipetting Module:
Single-channel/8-channel
(interchangeable) 

Maximum Volume:
50µl/200µl

 

Connection:
RS-232, USB2.00Hz, 100W

 

Size (WxDxH):
590 x 440 x 460 mm

 

Weight (Nett):
25 Kg

 

Trademarks: Beckman®, Biomek® 3000, Biomek® FX, Biomek® NX (Beckman Coulter Inc.); Roche®, LightCycler® 480 (Roche Group); DyNAmo™ (Finnzyme Oy); SYBR® (Molecular Probes Inc.); Invitrogen™ Platinum® (Invitrogen Corp.)

Code

Description

Quantity

4ti-2000

4ti-2200

4ti-2300

4ti-2303

4LAB™ 4-Position System

4LAB6™ 6-Position System

4LAB6™ 6-Position System with UV lamp and HEPA filter

4LAB6™ 6-Position System with UV lamp and HEPA filter, with active cooling and heating blocks

1

1

1

1

Pipetting Modules

4ti-2010

4ti-2020

4ti-2030

4ti-2040

1-50µl Single-Channel Pipetting Module

1-50µl 8-Channel Pipetting Module

10-200µl Single-Channel Pipetting Module

10-200µl 8-Channel Pipetting Module

1

1

1

1

Accessories

4ti-2051

4ti-2052

4ti-2053

4ti-2054

4ti-2055

4ti-2056

4ti-2057

4ti-2058

4ti-2059

4ti-2060

4ti-2061

4ti-2062

4ti-2063

4ti-2064

96-Tip Rack Adapter

Elevated 96-well PCR Plate Adapter

Elevated 384-well PCR Plate Adapter

1.5ml Tube Adapter (4x2)

2ml (3x2) and 5ml (1) Tube Adapter

Cooling Block for 96-well PCR Plates

Cooling Block for 384-well PCR Plates

Cooling Block for 1.5ml Tubes (4x2)

Cooling Block for 2ml (3x2) and 5ml (1) Tubes

20x1.5ml Tube Adapter

Used Tip Tray, Disposable

8-Channel Pipetting Reservoir

Deep-Well Plate Adapter

Reservoir (80ml)

1

1

1

1

1

1

1

1

1

1

10 Trays

1

1

20 Reservoirs

Pipette Tips

4ti-2100

4ti-2101

4ti-2104

4ti-2105

50μl Pipette Tip, Non-Sterile

200μl Pipette Tip, Non-Sterile

50µl Pipette Tip, Filtered, Sterile

200µl Pipette Tip, Filtered, Sterile

10x96

10x96

10x96

10x96

“40% reagent costs and 50% labour costs were saved by adopting the 4LAB™ and 384-well PCR microplates in our SNP assay project which extended to 1 year... ...the 4LAB™ provided the high accuracy and precision we needed.“

Director of the Contract Research Dept., 4LAB™ customer.

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